Fluorescence Microscopy Imaging Python Packages

ptufile

Read and write PicoQuant PTU and related files (PHU, PCK, PCO, PFS, PUS, PQRES, PQDAT, PQUNI, SPQR, and BIN).

sdtfile

Read Becker & Hickl SDT files.

lfdfiles

Laboratory for Fluorescence Dynamics (LFD) file formats.

liffile

Read Leica image files (LIF, LOF, XLIF, XLEF, XLCF, XLLF, and LIFEXT).

phasorpy

An open-source Python library for the analysis of luminescence lifetime and hyperspectral images using the phasor approach.

oiffile

Read Olympus image files (OIF and OIB).

fbdfile

Read FLIMbox data and related files (FBD, FBF, and FBS.XML).

psf

Point Spread Function calculations for fluorescence microscopy.

chebyfit

Fit exponential and harmonic functions using Chebyshev polynomials.

ams-bp

Advanced Microscopy Simulations - Create realistic fluorescence microscopy data!

obffile

Read Imspector object binary format files (OBF and MSR).

oirfile

Read Olympus/Evident OIR and POIR files.

tttrlib

File format agnostic low level, high performance API to read and process time-tagged-time resolved (TTTR) data for single-molecule and image spectroscopy)

domb-napari

napari plugin for analyzing fluorescence-labeled proteins redistribution

pathml

Tools for computational pathology

icsdll

Interface to the image correlation spectroscopy library ICSx64.dll.

efishent

RNA smFISH oligos/probes design tool

fuse-toolkit

FUSE toolkit supports fluorescent cell image alignment and analysis.

pyfrp

PyFRAP: A Python based FRAP analysis tool box

colicoords

Single-cell fluorescence microscopy data analysis

zetastitcher

ZetaStitcher is a tool designed to stitch large volumetric images such as those produced by Light-Sheet Fluorescence Microscopes.

birfi

Blind instrument response function identification from fluorescence decays

napari-lfdfiles

Laboratory for Fluorescence Dynamics (LFD) file formats.

lifffile

Read Leica image files (LIF, LOF, XLIF, XLEF, XLCF, XLLF, and LIFEXT).