Fluorescence Microscopy Imaging Python Packages

liffile

Read Leica image files (LIF, LOF, XLIF, XLCF, XLEF, and LIFEXT).

sdtfile

Read Becker & Hickl SDT files.

ptufile

Read and write PicoQuant PTU and related files (PHU, PCK, PCO, PFS, PUS, PQRES, PQDAT, PQUNI, SPQR, and BIN).

fbdfile

Read FLIMbox data and related files (FBD, FBF, and FBS.XML).

lfdfiles

Laboratory for Fluorescence Dynamics (LFD) file formats.

phasorpy

An open-source Python library for the analysis of luminescence lifetime and hyperspectral images using the phasor approach.

oiffile

Read Olympus image files (OIF and OIB).

chebyfit

Fit exponential and harmonic functions using Chebyshev polynomials.

psf

Point Spread Function calculations for fluorescence microscopy.

tttrlib

File format agnostic low level, high performance API to read and process time-tagged-time resolved (TTTR) data for single-molecule and image spectroscopy)

ams-bp

Advanced Microscopy Simulations - Create realistic fluorescence microscopy data!

pathml

Tools for computational pathology

efishent

RNA smFISH oligos/probes design tool

domb-napari

napari plugin for analyzing fluorescence-labeled proteins redistribution

icsdll

Interface to the image correlation spectroscopy library ICSx64.dll.

oirfile

Read Olympus/Evident OIR and POIR files.

obffile

Read Imspector object binary format files (OBF and MSR).

fuse-toolkit

FUSE toolkit supports fluorescent cell image alignment and analysis.

pyfrp

PyFRAP: A Python based FRAP analysis tool box

colicoords

Single-cell fluorescence microscopy data analysis

zetastitcher

ZetaStitcher is a tool designed to stitch large volumetric images such as those produced by Light-Sheet Fluorescence Microscopes.

birfi

Blind instrument response function identification from fluorescence decays

napari-sdtfile

Read Becker & Hickl SDT files.

napari-oiffile

Read Olympus image files (OIF and OIB).